A Narcissus mosaic viral vector system for protein expression and flavonoid production.

ABSTRACT:

Background

With the explosive numbers of sequences generated by next generation sequencing, the demand for high throughput screening to understand gene function has grown. Plant viral vectors have been widely used as tools in down-regulating plant gene expression. However, plant viral vectors can also express proteins in a very efficient manner and, therefore, can also serve as a valuable tool for characterizing proteins and their functions in metabolic pathways in planta.

Results

In this study, we have developed a Gateway®-based high throughput viral vector cloning system from Narcissus Mosaic Virus (NMV). Using the reporter genes of GFP and GUS, and the plant genes PAP1 (an R2R3 MYB which activates the anthocyanin pathway) and selenium-binding protein 1 (SeBP), we show tha

SUBMITTER: Zhang H 

PROVIDER: S-EPMC3728148 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature