Improving transcriptome assembly through error correction of high-throughput sequence reads.

ABSTRACT: The study of functional genomics, particularly in non-model organisms, has been dramatically improved over the last few years by the use of transcriptomes and RNAseq. While these studies are potentially extremely powerful, a computationally intensive procedure, the de novo construction of a reference transcriptome must be completed as a prerequisite to further analyses. The accurate reference is critically important as all downstream steps, including estimating transcript abundance are critically dependent on the construction of an accurate reference. Though a substantial amount of research has been done on assembly, only recently have the pre-assembly procedures been studied in detail. Specifically, several stand-alone error correction modules have been reported on and, while they have sh

SUBMITTER: Macmanes MD 

PROVIDER: S-EPMC3728768 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature