Molecular characterization of NDM-1 producing Enterobacteriaceae isolates in Singapore hospitals.

ABSTRACT:

Objective

In this study, we molecularly characterized 12 NDM-1 producing clinical Enterobacteriaceae (Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae) isolates that were part of a collection of non-carbapenem susceptible isolates obtained during a one-year period. These isolates were obtained from four local general hospitals in Singapore.

Methods

Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of β-lactamase encoding genes (bla) including bla NDM-1 and plasmid-mediated quinolone and aminoglycoside resistance determinants. Conjugation experiments were performed to determine the transferability of bla NDM-1. Isolate relatedness was determined by multilocus sequence typing (MLST).

Results

The isolates were completel

SUBMITTER: Teo J 

PROVIDER: S-EPMC3729072 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature