Physiological adaptations of Saccharomyces cerevisiae evolved for improved butanol tolerance.

ABSTRACT:

Background

Butanol is a chemical with potential uses as biofuel and solvent, which can be produced by microbial fermentation. However, the end product toxicity is one of the main obstacles for developing the production process irrespective of the choice of production organism. The long-term goal of the present project is to produce 2-butanol in Saccharomyces cerevisiae. Therefore, unraveling the toxicity mechanisms of solvents such as butanol and understanding the mechanisms by which tolerant strains of S. cerevisiae adapt to them would be an important contribution to the development of a bio-based butanol production process.

Results

A butanol tolerant S. cerevisiae was achieved through a series of sequential batch cultures with gradual increase of 2-butanol concentration. T

SUBMITTER: Ghiaci P 

PROVIDER: S-EPMC3729582 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature