Elevated carbon monoxide in the exhaled breath of mice during a systemic bacterial infection.

ABSTRACT: Blood is the specimen of choice for most laboratory tests for diagnosis and disease monitoring. Sampling exhaled breath is a noninvasive alternative to phlebotomy and has the potential for real-time monitoring at the bedside. Improved instrumentation has advanced breath analysis for several gaseous compounds from humans. However, application to small animal models of diseases and physiology has been limited. To extend breath analysis to mice, we crafted a means for collecting nose-only breath samples from groups and individual animals who were awake. Samples were subjected to gas chromatography and mass spectrometry procedures developed for highly sensitive analysis of trace volatile organic compounds (VOCs) in the atmosphere. We evaluated the system with experimental systemic infections o

SUBMITTER: Barbour AG 

PROVIDER: S-EPMC3729689 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature