Activation of SNAT1/SLC38A1 in human breast cancer: correlation with p-Akt overexpression.

ABSTRACT:

Background

SNAT1 is a subtype of the amino acid transport system A that has been implicated to play a potential role in cancer development and progression, yet its role in breast cancer remains unclear. In present study, we detected SNAT1 expression in breast cancers and explored its underlying mechanism in promoting breast carcinogenesis.

Methods

RT-PCR and Western blotting were performed to analyze the transcription and protein levels of SNAT1 in breast cancer cell lines and fresh tissues. Tissue microarray blocks containing breast cancer specimens obtained from 210 patients were constructed. Expression of SNAT1 in these specimens was analyzed using immunohistochemical studies. SNAT1 was down-regulated by SNAT1-shRNA in breast cancer cells and the functional significance w

SUBMITTER: Wang K 

PROVIDER: S-EPMC3729721 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature