Molecular characterization of the porcine group A rotavirus NSP2 and NSP5/6 genes from Sao Paulo State, Brazil, in 2011/12.

ABSTRACT: Rotaviruses are responsible for the acute diarrhea in various mammalian and avian species. The nonstructural proteins NSP2 and NSP5 are involved in the rotavirus replication and the formation of viroplasm, cytoplasmic inclusion bodies within which new viral particles morphogenesis and viral RNA replication occur. There are few studies on the genetic diversity of those proteins; thus this study aims at characterizing the diversity of rotavirus based on NSP2 and NSP5 genes in rotaviruses circulating in Brazilian pig farms. For this purpose, 63 fecal samples from pig farms located in six different cities in the São Paulo State, Brazil, were screened by nested RT-PCR. Seven strains had the partial nucleotide sequencing for NSP2, whereas in six, the total sequencing for NSP5. All were character

SUBMITTER: Barbosa BR 

PROVIDER: S-EPMC3730213 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature