Functional characterization of folate transport proteins in Staten's Seruminstitut rabbit corneal epithelial cell line.

ABSTRACT:

Purpose

The overall objective of this study was to investigate and characterize the expression of folate transport proteins in Staten's Seruminstitut rabbit corneal (SIRC) epithelial cell line.

Methods

[(3)H]Folic acid uptake was studied with respect to time, pH, temperature, sodium, and chloride ion dependency. Inhibition studies were conducted with structural analogs, vitamins, and metabolic inhibitors. [(3)H]Folic acid uptake was also determined with varying concentrations of cold folic acid. Uptake kinetics was studied in the presence of various modulators of intracellular regulatory pathways, protein kinases A and C (PKA and PKC), protein tyrosine kinase (PTK), and calcium-calmodulin modulators. Ex vivo corneal permeability studies were carried out with [(3)H]folic acid

SUBMITTER: Jwala J 

PROVIDER: S-EPMC3731148 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature