Post-translational modifications of pancreatic fluid proteins collected via the endoscopic pancreatic function test (ePFT).

ABSTRACT:

Background

Early diagnosis of chronic pancreatitis by mass spectrometry-based proteomics may result in therapies to retard or modify disease progression. We aimed to identify differences in posttranslational modifications (PTMs) in pancreatic fluid proteins from individuals with chronic pancreatitis (n=9) and non-pancreatitis controls (n=9).

Methods

We collected proteomic data from pancreatic fluid using mass spectrometry techniques. We performed database searches with emphasis on PTMs using ProteinPilot. We compared the frequency of specific PTMs in pancreatic fluid between cohorts and also to those identified in bile, gastroduodenal fluid, urine, and pancreatic duct and stellate cell lysates.

Results

We identified 97 PTMs in endoscopically-collected pancreatic fluid

SUBMITTER: Paulo JA 

PROVIDER: S-EPMC3731404 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature