Genome-wide profiling of human cap-independent translation-enhancing elements.

ABSTRACT: We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.

SUBMITTER: Wellensiek BP 

PROVIDER: S-EPMC3731418 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature