A hybrid approach for the automated finishing of bacterial genomes.

ABSTRACT: Advances in DNA sequencing technology have improved our ability to characterize most genomic diversity. However, accurate resolution of large structural events is challenging because of the short read lengths of second-generation technologies. Third-generation sequencing technologies, which can yield longer multikilobase reads, have the potential to address limitations associated with genome assembly. Here we combine sequencing data from second- and third-generation DNA sequencing technologies to assemble the two-chromosome genome of a recent Haitian cholera outbreak strain into two nearly finished contigs at >99.9% accuracy. Complex regions with clinically relevant structure were completely resolved. In separate control assemblies on experimental and simulated data for the canonical N1696

SUBMITTER: Bashir A 

PROVIDER: S-EPMC3731737 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature