Matrix metalloproteinases modulate ameboid-like migration of neutrophils through inflamed interstitial tissue.

ABSTRACT: In vitro studies suggest that leukocytes locomote in an ameboid fashion independently of pericellular proteolysis. Whether this motility pattern applies for leukocyte migration in inflamed tissue is still unknown. In vivo microscopy on the inflamed mouse cremaster muscle revealed that blockade of serine proteases or of matrix metalloproteinases (MMPs) significantly reduces intravascular accumulation and transmigration of neutrophils. Using a novel in vivo chemotaxis assay, perivenular microinjection of inflammatory mediators induced directional interstitial migration of neutrophils. Blockade of actin polymerization, but not of actomyosin contraction abolished neutrophil interstitial locomotion. Multiphoton laser scanning in vivo microscopy showed that the density of the interstitial collag

SUBMITTER: Lerchenberger M 

PROVIDER: S-EPMC3731931 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature