Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3'-untranslated regions.

ABSTRACT:

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Small secreted membrane vesicles called exosomes have recently attracted a great interest after the discovery that they transfer mRNA that can be translated into protein in recipient cells. Surprisingly, we found that for the majority of exosomal mRNAs only a fraction of their corresponding probes is detectable on the expression microarrays. Exosomal mRNA fragmentation is characterized with a specific structural pattern. The closer to the 3'-end of the transcript the fragments are localized, the larger fraction among the secreted RNAs they constitute. Since the 3'-ends of transcripts contain elements conferring subcellular localization of mRNA and are rich in miRNA-binding sites, exosomal RNA may act as competing RNA to regulate stability, localization and translation ac

SUBMITTER: Batagov AO 

PROVIDER: S-EPMC3732077 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature