Optimized metabolomic approach to identify uremic solutes in plasma of stage 3-4 chronic kidney disease patients.

ABSTRACT:

Background

Chronic kidney disease (CKD) is characterized by the progressive accumulation of various potential toxic solutes. Furthermore, uremic plasma is a complex mixture hampering accurate determination of uremic toxin levels and the identification of novel uremic solutes.

Methods

In this study, we applied (1)H-nuclear magnetic resonance (NMR) spectroscopy, following three distinct deproteinization strategies, to determine differences in the plasma metabolic status of stage 3-4 CKD patients and healthy controls. Moreover, the human renal proximal tubule cell line (ciPTEC) was used to study the influence of newly indentified uremic solutes on renal phenotype and functionality.

Results

Protein removal via ultrafiltration and acetonitrile precipitation are complementa

SUBMITTER: Mutsaers HA 

PROVIDER: S-EPMC3732267 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature