Paracrine regulation of theca androgen production by granulosa cells in the ovary.

ABSTRACT:

Objective

To test whether and to what extent inhibin mediates Cyp17 messenger RNA (mRNA) expression in theca cells (TCs) in response to FSH stimulation of granulosa cells (GCs).

Design

Ex vivo and in vitro experimental study.

Setting

University.

Animal(s)

Immature female Sprague Dawley rats.

Intervention(s)

Ovarian tissue explants and isolated theca cell preparations with or without GCs were treated with FSH, inhibin, inhibin antibody, or β-glycan antibody.

Main outcome measure(s)

As a key enzyme in androgen production, Cyp17 mRNA levels were measured by real-time reverse transcription-polymerase chain reaction.

Result(s)

After 24 hours, Cyp17 mRNA expression was dose-dependently increased by FSH in ovarian tissue explants and theca cells,

SUBMITTER: Hoang YD 

PROVIDER: S-EPMC3732568 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature