Quantification of the influence of protein-protein interactions on adsorbed protein structure and bioactivity.

ABSTRACT: While protein-surface interactions have been widely studied, relatively little is understood at this time regarding how protein-surface interaction effects are influenced by protein-protein interactions and how these effects combine with the internal stability of a protein to influence its adsorbed-state structure and bioactivity. The objectives of this study were to develop a method to study these combined effects under widely varying protein-protein interaction conditions using hen egg-white lysozyme (HEWL) adsorbed on silica glass, poly(methyl methacrylate), and polyethylene as our model systems. In order to vary protein-protein interaction effects over a wide range, HEWL was first adsorbed to each surface type under widely varying protein solution concentrations for 2h to saturate the

SUBMITTER: Wei Y 

PROVIDER: S-EPMC3732647 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature