Sequence and structural analysis of 3' untranslated region of hepatitis C virus, genotype 3a, from pakistani isolates.

ABSTRACT:

Background

Hepatitis C virus (HCV) is the cause of high morbidity and mortality worldwide, inflicting around one million people in Pakistan alone. The HCV genomic RNA harbors conserved structural elements that are indispensable for its replication. The 3' untranslated region (UTR) contains several of these elements essentially involved in regulating the major steps of the viral life cycle.

Objectives

Differences in regulatory elements of HCV may contribute towards differential infectivity of local isolates. The present study explicates sequence analysis and secondary structure prediction of HCV 3'UTR region of subtype 3a from Pakistan to characterize this particular region.

Patients and methods

HCV 3'UTR region was amplified, cloned and sequenced from five different p

SUBMITTER: Anjum S 

PROVIDER: S-EPMC3732663 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature