Computational design of orthogonal nucleoside kinases.

ABSTRACT: We report the computational enzyme design of an orthogonal nucleoside analog kinase for 3'-deoxythymidine. The best kinase variant shows an 8500-fold change in substrate specificity, resulting from a 4.6-fold gain in catalytic efficiency for the nucleoside analog and a 2000-fold decline for the native substrate thymidine.

SUBMITTER: Liu L 

PROVIDER: S-EPMC3733048 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature