Validation of ovarian cancer gene expression signatures for survival and subtype in formalin fixed paraffin embedded tissues.

ABSTRACT:

Introduction

Gene expression signatures have been identified for epithelial ovarian cancer survival (TCGA) and intrinsic subtypes (Tothill et al.). One obstacle to clinical translation is that these signatures were developed using frozen tissue, whereas usually only formalin-fixed, paraffin embedded (FFPE) tissue is available. The aim of this study was to determine if gene expression signatures can be translated to fixed archival tissues.

Methods

RNA extracted from FFPE sections from 240 primary ovarian cancers was analyzed by DASL on Illumina BeadChip arrays. Concordance of expression at the individual gene level was assessed by comparing array data from the same cancers (30 frozen samples analyzed on Affymetrix arrays versus FFPE DASL).

Results

The correlation betwe

SUBMITTER: Sfakianos GP 

PROVIDER: S-EPMC3733243 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature