MELTING, a flexible platform to predict the melting temperatures of nucleic acids.

ABSTRACT:

Background

Computing accurate nucleic acid melting temperatures has become a crucial step for the efficiency and the optimisation of numerous molecular biology techniques such as in situ hybridization, PCR, antigene targeting, and microarrays. MELTING is a free open source software which computes the enthalpy, entropy and melting temperature of nucleic acids. MELTING 4.2 was able to handle several types of hybridization such as DNA/DNA, RNA/RNA, DNA/RNA and provided corrections to melting temperatures due to the presence of sodium. The program can use either an approximative approach or a more accurate Nearest-Neighbor approach.

Results

Two new versions of the MELTING software have been released. MELTING 4.3 is a direct update of version 4.2, integrating newly available ther

SUBMITTER: Dumousseau M 

PROVIDER: S-EPMC3733425 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature