Analysis of hairpin RNA transgene-induced gene silencing in Fusarium oxysporum.

ABSTRACT:

Background

Hairpin RNA (hpRNA) transgenes can be effective at inducing RNA silencing and have been exploited as a powerful tool for gene function analysis in many organisms. However, in fungi, expression of hairpin RNA transcripts can induce post-transcriptional gene silencing, but in some species can also lead to transcriptional gene silencing, suggesting a more complex interplay of the two pathways at least in some fungi. Because many fungal species are important pathogens, RNA silencing is a powerful technique to understand gene function, particularly when gene knockouts are difficult to obtain. We investigated whether the plant pathogenic fungus Fusarium oxysporum possesses a functional gene silencing machinery and whether hairpin RNA transcripts can be employed to effectively

SUBMITTER: Schumann U 

PROVIDER: S-EPMC3733888 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature