Empirical assessment of competitive hybridization and noise in ultra high density canine tiling arrays.

ABSTRACT:

Background

In addition to probe sequence characteristics, noise in hybridization array data is thought to be influenced by competitive hybridization between probes tiled at high densities. Empirical evaluation of competitive hybridization and an estimation of what other non-sequence related features might affect noisy data is currently lacking.

Results

A high density array was designed to a 1.5 megabase region of the canine genome to explore the potential for probe competition to introduce noise. Multivariate assessment of the influence of probe, segment and design characteristics on hybridization intensity demonstrate that whilst increased density significantly depresses fluorescence intensities, this effect is largely consistent when an ultra high density offset is applied

SUBMITTER: Willet CE 

PROVIDER: S-EPMC3733988 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature