MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS).

ABSTRACT:

Background

MicroRNA are small noncoding RNA molecules that are involved in the control of gene expression. To investigate the role of microRNA in multiple sclerosis (MS), we performed genome-wide expression analyses of mRNA and microRNA in T-cells from MS patients and controls.

Methods

Heparin-anticoagulated peripheral blood was collected from MS-patients and healthy controls followed by isolation of T-cells. MicroRNA and RNA from T-cells was prepared and hybridized to Affymetrix miR 2.0 array and Affymetrix U133Plus 2.0 Human Genome array (Santa Clara, CA), respectively. Verifications were performed with real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA).

Results

We identified 2,452 differentially expressed genes and 21 differenti

SUBMITTER: Jernas M 

PROVIDER: S-EPMC3734042 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature