Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.

ABSTRACT: Microtubule-based transport mediates the sorting and dispersal of many cellular components and pathogens. However, the mechanisms by which motor complexes are recruited to and regulated on different cargos remain poorly understood. Here we describe a large-scale biochemical screen for novel factors associated with RNA localization signals mediating minus end-directed mRNA transport during Drosophila development. We identified the protein Lissencephaly-1 (Lis1) and found that minus-end travel distances of localizing transcripts are dramatically reduced in lis1 mutant embryos. Surprisingly, given its well-documented role in regulating dynein mechanochemistry, we uncovered an important requirement for Lis1 in promoting the recruitment of dynein and its accessory complex dynactin to RNA locali

SUBMITTER: Dix CI 

PROVIDER: S-EPMC3734092 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature