Complete genomic sequence of Epstein-Barr virus in nasopharyngeal carcinoma cell line C666-1.

ABSTRACT:

Background

Nasopharyngeal carcinoma is a distinct type of head and neck cancer which is consistently associated with Epstein-Barr virus (EBV). The C666-1 cell line is the only in vitro native EBV-infected NPC cell model commonly used for study of the viral-host interaction. Nevertheless, the complete EBV genome sequence in this in vitro EBV-infected NPC model has not been characterized.

Objective

To determine the complete EBV genome sequence in C666-1 cells.

Methods

The C666-1 genome was sequenced by 100-bases pair-end massive parallel sequencing. Bioinformatics analysis was performed to extract the EBV sequences and construct an EBV consensus sequence map. PCR amplification and Sanger DNA sequencing were used for sequence validation and gap filling. A phylogenetic an

SUBMITTER: Tso KK 

PROVIDER: S-EPMC3734220 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature