Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.

ABSTRACT: Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and recombinant DNA studies among rosaceous crops. Functional genomic and molecular studies involve relative quantification of gene expression under experimental conditions of interest. Accuracy and reliability are dependent upon the choice of an optimal reference control transcript. There is no information available on validated endogenous reference genes for use in studies testing strawberry-pathogen interactions. Thirteen potential pre-selected strawberry reference genes were tested against different tissues, strawberry cultivars, biotic stresses, ripening and senescent conditions, and SA/JA treatments. Evaluation of reference candidate's suitability was analyzed by five different methodologies, and inf

SUBMITTER: Amil-Ruiz F 

PROVIDER: S-EPMC3734262 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature