Identification of a metabolizing enzyme in human kidney by proteomic correlation profiling.

ABSTRACT: Molecular identification of endogenous enzymes and biologically active substances from complex biological sources remains a challenging task, and although traditional biochemical purification is sometimes regarded as outdated, it remains one of the most powerful methodologies for this purpose. While biochemical purification usually requires large amounts of starting material and many separation steps, we developed an advanced method named "proteomic correlation profiling" in our previous study. In proteomic correlation profiling, we first fractionated biological material by column chromatography, and then calculated each protein's correlation coefficient between the enzyme activity profile and protein abundance profile determined by proteomics technology toward fractions. Thereafter, we co

SUBMITTER: Sakurai H 

PROVIDER: S-EPMC3734587 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature