Discovery and mass spectrometric analysis of novel splice-junction peptides using RNA-Seq.

ABSTRACT: Human proteomic databases required for MS peptide identification are frequently updated and carefully curated, yet are still incomplete because it has been challenging to acquire every protein sequence from the diverse assemblage of proteoforms expressed in every tissue and cell type. In particular, alternative splicing has been shown to be a major source of this cell-specific proteomic variation. Many new alternative splice forms have been detected at the transcript level using next generation sequencing methods, especially RNA-Seq, but it is not known how many of these transcripts are being translated. Leveraging the unprecedented capabilities of next generation sequencing methods, we collected RNA-Seq and proteomics data from the same cell population (Jurkat cells) and created a bioinfo

SUBMITTER: Sheynkman GM 

PROVIDER: S-EPMC3734590 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature