Minicircle DNA is superior to plasmid DNA in eliciting antigen-specific CD8+ T-cell responses.

ABSTRACT: Clinical trials reveal that plasmid DNA (pDNA)-based gene delivery must be improved to realize its potential to treat human disease. Current pDNA platforms suffer from brief transgene expression, primarily due to the spread of transcriptionally repressive chromatin initially deposited on plasmid bacterial backbone sequences. Minicircle (MC) DNA lacks plasmid backbone sequences and correspondingly confers higher levels of sustained transgene expression upon delivery, accounting for its success in preclinical gene therapy models. In this study, we show for the first time that MC DNA also functions as a vaccine platform. We used a luciferase reporter transgene to demonstrate that intradermal delivery of MC DNA, relative to pDNA, resulted in significantly higher and persistent levels of lucife

SUBMITTER: Dietz WM 

PROVIDER: S-EPMC3734653 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature