Modeling of brain metabolism and pyruvate compartmentation using (13)C NMR in vivo: caution required.

ABSTRACT: Two variants of a widely used two-compartment model were prepared for fitting the time course of [1,6-(13)C2]glucose metabolism in rat brain. Features common to most models were included, but in one model the enrichment of the substrates entering the glia and neuronal citric acid cycles was allowed to differ. Furthermore, the models included the capacity to analyze multiplets arising from (13)C spin-spin coupling, known to improve parameter estimates in heart. Data analyzed were from a literature report providing time courses of [1,6-(13)C2]glucose metabolism. Four analyses were used, two comparing the effect of different pyruvate enrichment in glia and neurons, and two for determining the effect of multiplets present in the data. When fit independently, the enrichment in glial pyruvate wa

SUBMITTER: Jeffrey FM 

PROVIDER: S-EPMC3734769 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature