Screening of osteoprotegerin-related feature genes in osteoporosis and functional analysis with DNA microarray.

ABSTRACT:

Background

Osteoporosis affects 200 million people worldwide and places an enormous economic burden on society. We aim to identify the feature genes that are related to osteoprotegerin in osteoporosis and to perform function analysis with DNA microarray from human bone marrow.

Methods

We downloaded the gene expression profile GSE35957 from Gene Expression Omnibus database including nine gene chips from bone marrow mesenchymal stem cells of five osteoporotic and four non-osteoporotic subjects. The differentially expressed genes between normal and disease samples were identified by LIMMA package in R language. The interactions among the osteoprotegerin gene (OPG) and differentially expressed genes were searched and visualized by Cytoscape. MCODE and Bingo were used to perform

SUBMITTER: Wu X 

PROVIDER: S-EPMC3735399 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature