Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling.

ABSTRACT:

Background

Although there is extensive evidence for the amoeboid invasiveness of cancer cells in vitro, much less is known about the role of amoeboid invasiveness in metastasis and the importance of Rho/ROCK/MLC signaling in this process.

Results

We analyzed the dependence of amoeboid invasiveness of rat and chicken sarcoma cells and the metastatic activity of chicken cells on individual elements of the Rho/ROCK/MLC pathway. In both animal models, inhibition of Rho, ROCK or MLC resulted in greatly decreased cell invasiveness in vitro, while inhibition of extracellular proteases using a broad spectrum inhibitor did not have a significant effect. The inhibition of both Rho activity and MLC phosphorylation by dominant negative mutants led to a decreased capability of chicken sa

SUBMITTER: Kosla J 

PROVIDER: S-EPMC3735423 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature