Metabolic alterations in mammary cancer prevention by withaferin A in a clinically relevant mouse model.

ABSTRACT:

Background

Efficacy of withaferin A (WA), an Ayurvedic medicine constituent, for prevention of mammary cancer and its associated mechanisms were investigated using mouse mammary tumor virus-neu (MMTV-neu) transgenic model.

Methods

Incidence and burden of mammary cancer and pulmonary metastasis were scored in female MMTV-neu mice after 28 weeks of intraperitoneal administration with 100 µg WA (three times/week) (n = 32) or vehicle (n = 29). Mechanisms underlying mammary cancer prevention by WA were investigated by determination of tumor cell proliferation, apoptosis, metabolomics, and proteomics using plasma and/or tumor tissues. Spectrophotometric assays were performed to determine activities of complex III and complex IV. All statistical tests were two-sided.

Results

SUBMITTER: Hahm ER 

PROVIDER: S-EPMC3735460 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature