Imaging the intact mouse cornea using coherent anti-stokes Raman scattering (CARS).

ABSTRACT:

Purpose

The aim of this study was to image the cellular and noncellular structures of the cornea and limbus in an intact mouse eye using the vibrational oscillation of the carbon-hydrogen bond in lipid membranes and autofluorescence as label-free contrast agents.

Methods

Freshly enucleated mouse eyes were imaged using two nonlinear optical techniques: coherent anti-Stokes Raman scattering (CARS) and two-photon autofluorescence (TPAF). Sequential images were collected through the full thickness of the cornea and limbal regions. Line scans along the transverse/sagittal axes were also performed.

Results

Analysis of multiple CARS/TPAF images revealed that corneal epithelial and endothelial cells could be identified by the lipid-rich plasma membrane CARS signal. The fluore

SUBMITTER: Ammar DA 

PROVIDER: S-EPMC3736760 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature