Development of microsatellite markers by transcriptome sequencing in two species of Amorphophallus (Araceae).

ABSTRACT:

Background

Amorphophallus is a genus of perennial plants widely distributed in the tropics or subtropics of West Africa and South Asia. Its corms contain a high level of water-soluble glucomannan; therefore, it has long been used as a medicinal herb and food source. Genetic studies of Amorphophallus have been hindered by a lack of genetic markers. A large number of molecular markers are required for genetic diversity study and improving disease resistance in Amorphophallus. Here, we report large scale of transcriptome sequencing of two species: Amorphophallus konjac and Amorphophallus bulbifer using deep sequencing technology, and microsatellite (SSR) markers were identified based on these transcriptome sequences.

Results

cDNAs of A. konjac and A. bulbifer were sequenced usi

SUBMITTER: Zheng X 

PROVIDER: S-EPMC3737116 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature