Discovery and validation of DNA hypomethylation biomarkers for liver cancer using HRM-specific probes.

ABSTRACT: Poor prognosis of hepatocellular carcinoma (HCC) associated with late diagnosis necessitates the development of early diagnostic biomarkers. We have previously delineated the landscape of DNA methylation in HCC patients unraveling the importance of promoter hypomethylation in activation of cancer- and metastasis-driving genes. The purpose of the present study was to test the feasibility that genes that are hypomethylated in HCC could serve as candidate diagnostic markers. We use high resolution melting analysis (HRM) as a simple translatable PCR-based method to define methylation states in clinical samples. We tested seven regions selected from the shortlist of genes hypomethylated in HCC and showed that HRM analysis of several of them distinguishes methylation states in liver cancer speci

SUBMITTER: Stefanska B 

PROVIDER: S-EPMC3737236 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature