Establishment and characterization of primary glioblastoma cell lines from fresh and frozen material: a detailed comparison.

ABSTRACT:

Background

Development of clinically relevant tumor model systems for glioblastoma multiforme (GBM) is important for advancement of basic and translational biology. High molecular heterogeneity of GBM tumors is well recognized, forming the rationale for molecular tests required before administration of several of the novel therapeutics rapidly entering the clinics. One model that has gained wide acceptance is the primary cell culture model. The laborious and time consuming process is rewarded with a relative high success rate (about 60%). We here describe and evaluate a very simple cryopreservation procedure for GBM tissue prior to model establishment that will considerably reduce the logistic complexity.

Methods

Twenty-seven GBM samples collected ad hoc were prepared for pr

SUBMITTER: Mullins CS 

PROVIDER: S-EPMC3737284 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature