STAT3 upregulates miR-92a to inhibit RECK expression and to promote invasiveness of lung cancer cells.

ABSTRACT:

Background

Signal transducer and activator of transcription 3 (STAT3) activation is frequently found in human lung cancer and is associated with increased metastasis and reduced survival. How STAT3 enhances invasiveness is unclear.

Methods

The expression of microRNAs and target genes was measured by real-time RT-PCR. Protein level was studied by western blotting. Luciferase reporter assay was used to confirm the direct targeting of microRNAs. Gelatin zymography was used to study matrix metalloproteinase (MMP) activity. Transwell assay was used to investigate cell migration and invasion.

Results

Enforced expression of STAT3 decreases the endogenous MMP inhibitor RECK protein but not mRNA level in H460 cells. Conversely, STAT3 inhibitor S3I-201 increases RECK protein in

SUBMITTER: Lin HY 

PROVIDER: S-EPMC3738132 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature