Functionally relevant microsatellite markers from chickpea transcription factor genes for efficient genotyping applications and trait association mapping.

ABSTRACT: We developed 1108 transcription factor gene-derived microsatellite (TFGMS) and 161 transcription factor functional domain-associated microsatellite (TFFDMS) markers from 707 TFs of chickpea. The robust amplification efficiency (96.5%) and high intra-specific polymorphic potential (34%) detected by markers suggest their immense utilities in efficient large-scale genotyping applications, including construction of both physical and functional transcript maps and understanding population structure. Candidate gene-based association analysis revealed strong genetic association of TFFDMS markers with three major seed and pod traits. Further, TFGMS markers in the 5' untranslated regions of TF genes showing differential expression during seed development had higher trait association potential. The

SUBMITTER: Kujur A 

PROVIDER: S-EPMC3738162 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature