Local cytoskeletal and organelle interactions impact molecular-motor- driven early endosomal trafficking.

ABSTRACT:

Background

In the intracellular environment, motor-driven cargo must navigate a dense cytoskeletal network among abundant organelles.

Results

We investigated the effects of the crowded intracellular environment on early endosomal trafficking. Live-cell imaging of an endosomal cargo (endocytosed epidermal growth factor-conjugated quantum dots) combined with high-resolution tracking was used to analyze the heterogeneous motion of individual endosomes. The motile population of endosomes moved toward the perinuclear region in directed bursts of microtubule-based, dynein-dependent transport interrupted by longer periods of diffusive motion. Actin network density did not affect motile endosomes during directed runs or diffusive interruptions. Simultaneous two-color imaging was use

SUBMITTER: Zajac AL 

PROVIDER: S-EPMC3738301 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature