Identification of Trypanosome proteins in plasma from African sleeping sickness patients infected with T. b. rhodesiense.

ABSTRACT: Control of human African sleeping sickness, caused by subspecies of the protozoan parasite Trypanosoma brucei, is based on preventing transmission by elimination of the tsetse vector and by active diagnostic screening and treatment of infected patients. To identify trypanosome proteins that have potential as biomarkers for detection and monitoring of African sleeping sickness, we have used a 'deep-mining" proteomics approach to identify trypanosome proteins in human plasma. Abundant human plasma proteins were removed by immunodepletion. Depleted plasma samples were then digested to peptides with trypsin, fractionated by basic reversed phase and each fraction analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This sample processing and analysis method enabled identificat

SUBMITTER: Eyford BA 

PROVIDER: S-EPMC3738533 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature