Development of a new approach to aid in visual identification of murine iPS colonies using a fuzzy logic decision support system.

ABSTRACT: The a priori identification of induced pluripotent stem cells remains a challenge. Being able to quickly identify the most embryonic stem cell-similar induced pluripotent stem cells when validating results could help to reduce costs and save time. In this context, tools based on non-classic logic can be useful in creating aid-systems based on visual criteria. True colonies when viewed at 100x magnification have been found to have the following 3 characteristics: a high degree of border delineation, a more uniform texture, and the absence of a cracked texture. These visual criteria were used for fuzzy logic modeling. We investigated the possibility of predicting the presence of alkaline phosphatase activity, typical of true induced pluripotent stem cell colonies, after 25 individuals, with

SUBMITTER: Bassaneze V 

PROVIDER: S-EPMC3738584 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature