Differences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.

ABSTRACT: The host genotype has been proposed to contribute to individually composed bacterial communities in the gut. To provide deeper insight into interactions between gut bacteria and host, we associated germ-free C3H and C57BL/10 mice with intestinal bacteria from a C57BL/10 donor mouse. Analysis of microbiota similarity between the animals with denaturing gradient gel electrophoresis revealed the development of a mouse strain-specific microbiota. Microarray-based gene expression analysis in the colonic mucosa identified 202 genes whose expression differed significantly by a factor of more than 2. Application of bioinformatics tools demonstrated that functional terms including signaling/secretion, lipid degradation/catabolism, guanine nucleotide/guanylate binding and immune response were signif

SUBMITTER: Brodziak F 

PROVIDER: S-EPMC3739790 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature