Inferring phylogeny and introgression using RADseq data: an example from flowering plants (Pedicularis: Orobanchaceae).

ABSTRACT: Phylogenetic relationships among recently diverged species are often difficult to resolve due to insufficient phylogenetic signal in available markers and/or conflict among gene trees. Here we explore the use of reduced-representation genome sequencing, specifically in the form of restriction-site associated DNA (RAD), for phylogenetic inference and the detection of ancestral hybridization in non-model organisms. As a case study, we investigate Pedicularis section Cyathophora, a systematically recalcitrant clade of flowering plants in the broomrape family (Orobanchaceae). Two methods of phylogenetic inference, maximum likelihood and Bayesian concordance, were applied to data sets that included as many as 40,000 RAD loci. Both methods yielded similar topologies that included two major clade

SUBMITTER: Eaton DA 

PROVIDER: S-EPMC3739883 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature