A prototype tobacco-associated oral squamous cell carcinoma classifier using RNA from brush cytology.

ABSTRACT:

Background

Oral cancer in the form of squamous cell carcinoma (OSCC) is typically detected in advanced stages when treatment is complex and may not be curative. The need for surgical biopsy may contribute to delays in diagnosis and impede early detection. Multiple studies of RNA from surgically obtained tumor samples have revealed many genes differentially expressed with this disease. We sought to determine whether the identified mRNAs could be used as markers by a non-invasive detection system for OSCC using RNA from brush cytology.

Methods

Levels of mRNAs from 21 genes known to be differentially expressed in head and neck squamous cell carcinoma surgical samples, compared with controls, were shown to be quantifiable in oral brush cytology samples. These mRNAs were quantifi

SUBMITTER: Kolokythas A 

PROVIDER: S-EPMC3740027 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature