NTHi induction of Cxcl2 and middle ear mucosal metaplasia in mice.

ABSTRACT:

Objectives/hypothesis

Chronic otitis media (COM) develops after sustained inflammation and is characterized by secretory middle ear epithelial metaplasia and effusion, most frequently mucoid. Nontypeable Haemophilus influenzae (NTHi), the most common acute otitis media (OM) pathogen, is known to activate inflammation and mucin expression in vitro and in animal models of OM. The goals of this study were to examine histopathological and expression profiling epithelial effects of NTHi challenge in murine middle ears.

Study design

In vitro and in vivo murine model of OM.

Methods

Weekly transtympanic inoculation of Balb/c mice with 300 μg/ml of NTHi lysates versus saline was performed. Histopathologic analysis was carried out at 4 weeks. Expression microarray analysis was

SUBMITTER: Preciado D 

PROVIDER: S-EPMC3740030 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature