Spin-labeled pH-sensitive phospholipids for interfacial pKa determination: synthesis and characterization in aqueous and micellar solutions.

ABSTRACT: The synthesis and characterization of spin-labeled phospholipids (SLP)--derivatives of 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol (PTE)--with pH-reporting nitroxides that are covalently attached to the lipid's polar headgroup are being reported. Two lipids were synthesized by reactions of PTE with thiol-specific, pH-sensitive methanethiosulfonate spin labels methanethiosulfonic acid S-(1-oxyl-2,2,3,5,5-pentamethylimidazolidin-4-ylmethyl) ester (IMTSL) and S-4-(4-(dimethylamino)-2-ethyl-5,5-dimethyl-1-oxyl-2,5-dihydro-1H-imidazol-2-yl)benzyl methanethiosulfonate (IKMTSL). The pKa values of the IMTSL-PTE lipid measured by EPR titration in aqueous buffer/isopropyl alcohol solutions of various compositions were found to be essentially the same (pKa approximately 2.35), indicating that in

SUBMITTER: Voinov MA 

PROVIDER: S-EPMC3740190 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature