Filaggrin-dependent secretion of sphingomyelinase protects against staphylococcal α-toxin-induced keratinocyte death.

ABSTRACT:

Background

The skin of patients with atopic dermatitis (AD) has defects in keratinocyte differentiation, particularly in expression of the epidermal barrier protein filaggrin. AD skin lesions are often exacerbated by Staphylococcus aureus-mediated secretion of the virulence factor α-toxin. It is unknown whether lack of keratinocyte differentiation predisposes to enhanced lethality from staphylococcal toxins.

Objective

We investigated whether keratinocyte differentiation and filaggrin expression protect against cell death induced by staphylococcal α-toxin.

Methods

Filaggrin-deficient primary keratinocytes were generated through small interfering RNA gene knockdown. RNA expression was determined by using real-time PCR. Cell death was determined by using the lactate dehy

SUBMITTER: Brauweiler AM 

PROVIDER: S-EPMC3742335 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature