Characterization of differential protein tethering at the plasma membrane in response to epidermal growth factor signaling.

ABSTRACT: Physical tethering of membrane proteins to the cortical actin cytoskeleton provides functional organization to the plasma membrane and contributes to diverse cellular processes including cell signaling, vesicular trafficking, endocytosis, and migration. For these processes to occur, membrane protein tethering must be dynamically regulated in response to environmental cues. In this study, we describe a novel biochemical scheme for isolating the complement of plasma membrane proteins that are physically tethered to the actin cytoskeleton. We utilized this method in combination with tandem liquid chromatography/mass spectrometry (LC-MS/MS) to demonstrate that cytoskeletal tethering of membrane proteins is acutely regulated by epidermal growth factor (EGF) in normal human kidney (HK2) cells. O

SUBMITTER: Looyenga BD 

PROVIDER: S-EPMC3742339 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature