Gliomas promote immunosuppression through induction of B7-H1 expression in tumor-associated macrophages.

ABSTRACT:

Purpose

Gliomas are known to induce local and systemic immunosuppression, inhibiting T-cell-mediated cytotoxic responses to tumor growth. Tumor-associated macrophages are a significant component of the immune infiltrate in gliomas and may express immunosuppressive surface ligands, such as B7-H1.

Experimental design

Tumor and peripheral blood samples from patients with glioblastoma (GBM) were analyzed by flow cytometry to evaluate the expression of B7-H1 in circulating and tumor-infiltrating macrophages. Human monocytes from healthy patients were stimulated with conditioned media from glioma cells to evaluate B7-H1 expression. Production of interleukin (IL)-10 by stimulated monocytes was measured by ELISA, and stimulation with IL-10 alone was evaluated for the ability to indu

SUBMITTER: Bloch O 

PROVIDER: S-EPMC3742575 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature